1E.Z.N.A.® Total RNA Kit ITable of ContentsIntroduction....................................................................2Illustrated Protocol........................................................ .3Kit Contents/Storage and Stability................................4Preparing Reagents.........................................................5Recommended Settings................................................ 6Quantification of RNA......................................................7Disruption and Homogenization....................................8Animal Cell Protocol ....................................................... 10Animal Tissue Protocol .................................................13Spin/Vacuum Manifold Protocol...................................16DNase Digestion Protocol ............................................18Troubleshooting Guide.................................................. 20Ordering.........................................................................22Manual Revision: October 2009Innovations in nucleic acid isolation2Introdu ctionThe E.Z.N.A.® RNA family of products is an innovative system that radically simplifies the extraction and purification of RNA from a variety of sources. The key to this system is that it uses the reversible binding properties of the HiBind Matrix in combination with the speed of mini-column spin technology thereby permitting single or multiple simultaneous processing of samples. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the E.Z.N.A.® RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.The...
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