DDDDDDDDDDDDDDDDDDDDDDDDDDhUADDDDDDDDDDD2DDDDDDDDDDDDDDDDDDD90%4,(humanurattransporter,hUAT)/(humanurateaniontransporter1,hURAT1)(humanorganicaniontransporter,hOAT1)hOAT3hUAT,hURAT1,hOAT1hOAT3hUAThUAT3PPARPPAR-ARBPPAR-UATPPARUAT()hURAT1hOATIhOAT3UATUAT12-4ARBARB(PPAR)45a-SMATGF-plCAT悶啟in3MH)p-catinijUAT■TGF-pl■a-SMAWesternBlotGAPDHTGF-PhUATCAFDH■1UAT■TGf-piUATTGF-1WesternBlot6DDDDDDDDDDD1.通过体内实验，观察替米沙坦对痛风性肾病患者SUA、SCr、PPAR-丫、NO表达的影响，明确替米沙坦降尿酸的作用及机制。2.通过体外实验，观察人肾小管上皮细胞（HK-2）尿酸盐转运蛋白hUAT的表达及定位。探讨在正常及高尿酸条件下HK-2上hUAT、PPAR-丫、NO、CO-X2、MCP-1、TGF-pl的表达情况，观察替米沙坦、罗格列酮及PPAR-Y阻断剂HL005干预后其表达变化,进一步明确替米沙坦降尿酸及保护肾脏的机制。7DDDDDDDDDDDDDDDDDDDDDDDDDDDD2014D12DD2016D12DD8DDDDDDD1.2.hUAT2014.12-2015.22015.3-2016.1HK-2hUATHK-2hUATPPAR-NOCO-X2MCP-1TGF-1PPAR-HL0052015.3-2016.12100SUASCrPPAR-NO9DDDDDDDDDDDDDDDDDDD
