光照和激素对耐热型大白菜游离小孢子培养的影响X盛鹏1,张秀荣2,岳艳玲1XX,李晶晶1,邓静11.云南农业大学园林园艺学院,昆明650201;2.松原市前郭县白依拉嘎乡农业技术推广站,前郭131113摘要:将分离纯化的小孢子悬浮培养在添加0,011,012,013,015,110mg/L的NAA和0,0105,011,012,013,015,110mg/L6-BA的NLN培养基中,在A(3000lx光培养)、B(10d暗培养再3000lx光培养)、C(15d暗培养再3000lx光培养)、D(ck,暗培养)条件下进行培养,研究光照和激素对耐热型大白菜/夏将军0游离小孢子成胚及胚状体发育的影响。结果表明:011mg/L6-BA能显著促进胚状体的发生,使胚产量达到最高,为206个。同时附加012mg/LNAA不但保证有较高的胚产量(205个),还可促进胚状体发育,子叶胚及萌发胚可达3317%,畸形胚比例下降到1411%;暗培养能促进成胚,D处理成胚量高,达149个,光培养则明显抑制成胚,但可促进胚状体发育,提高成苗率,其中以B处理成胚成苗效果较好,成胚108个,成苗率145%。关键词:耐热型大白菜;游离小孢子;光照;激素;成胚;成苗中图分类号:S63411文献标识码:A文章编号:1000-5684(2011)06-0649-05DOI:CNKI:22-1100/S.20110707.1624.003网络出版地址:http://www.cnki.net/kcms/detail/22.1100.S.20110707.1624.003.html引文格式:盛鹏,张秀荣,岳艳玲,等1光照和激素对耐热型大白菜游离小孢子培养的影响[J].吉林农业大学学报,2011,33(6):649-653.EffectofDifferentLightandHormoneTreatmentsonIsolatedM-icrosporeCulturesofHeat-ToleranceChineseCabbageSHENGPeng1,ZHANGXiu-rong2,YUEYan-ling1,LIJing-jing1,DENGJing11.CollegeofLandscapeandHorticulture,YunnanAgriculturalUniversity,Kunming650201,China;2.QianguoBaiyilagaTownAgro-TechExtensionStationofSongyuanCity,Qianguo131113,ChinaAbstract:0,011,012,013,015and110mg/LNAAand0,0105,011,012,013and015,110mg/L6-BAwereaddedtoNLNmedia,andA-3000lxlight,B-10ddarkthen3000lxlight,C-15ddarkthen3000lxlightandD-darkwereappliedtoculturingisolatedmicrosporeofhea-ttoleranceChinesecabbage-Xiajiangjiuinordertostudytheeffectoflightandhormoneonthegrowthandinductionofmicrospore-derivedembryos.Theresultsshowed:011mg/L6-BAcouldimproveinductionofm-icrospor-ederivedembryos.Thehighestyield,206embryos,wasobtained.Atthesametimeadding012mg/LNAAcouldnotonlyinducehigherembryos,205embryos,butalsopromotegrowthofem-bryos.CotylednaryandGerminatedembryoincreasedto3317%andabnormalembryodecreasedto1411%.Darkculturecouldimproveinductionofmicrospore-derivedembryosandDtreatobtained149XXX通讯作者基金项目:云南省自然科学基金项目(2006C0032Q)作者简介:盛鹏,男,硕士,从事蔬菜遗传育种工作。收稿日期:2010-05-31网络出版时间:2011-07-0716:24吉林农业大学学报2011,33(6):649~653http://xuebao.jlau.edu.cnJournalofJilinAgriculturalUniversityE-mail:jlndxb@vip.sina.comembryos.Lightculture,however,decreasedembryoyieldbutimprovedthegrowthofembryosandregen-eratedplantlet.BetterBtreatinduced108embryosandrateofregeneratedplantletincreasedto145%.Keywords:hea-ttoleranceChinesecabbage;isolatedmicrospore;light;hormone;inductionofm-icrospore-derivedembryo;regeneratedplantlet自Sato等[1]利用游离小孢子培养技术在国际上首次成功获得大白菜小孢子胚和单倍体再生植株以来,芸薹属游离小孢子培养技术已得到不断完善[2-4],广泛应用于诱变和突变体筛选、基因转化等研究,通过花粉培养建立的双单倍体群体还是分子标记和基因图谱的理想材料。作为一种在单细胞水平上获得双单倍体纯系的方法,更是在大白菜优势育种上得到广泛关注[5-7]。游离小孢子成胚诱导及植株再生受众多因素影响[8-9],1989年Gland等[10]在甘蓝型油菜小孢子培养中发现,添加NAA可促进胚的发育,有利植株再生。Joao[11]在花椰菜和青花菜小孢子培养的研究中,也证实了添加细胞分裂素可以促进小孢子向胚的分化。在大白菜游离小孢子的培养研究中,Sato[1]、Li...
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