AgriculturalScience＆Technology，2011，12(2)：237—240Copyright⑥2011，InformationInstituteofHAAS．AllrightsreservedPlantProtectionPreIlminaryStudyonGarlicRootExudatesInfluencestotheGrowthofPomegranateWiltPathogen(Ceratocystisfimbriata)andBacillussubtilisTANGDong·sheng，WANGBin，MAOZhong-shun，HEXia-hongKeyLaboratoryforAgro—biodiversityandPestcontrolofMinistryofEducation，YunnanAgriculturalUniversity，Kunming650201Abstractfohiectives]ThispaperaimstoexplorethepossibilitytointercropgarlicwithpomegranatetreetocontroIpomegranatewilt．IMeth-ods1Rootexudatesofgarliciscultivatedin1／5concentrationofMSsolutionanddistilledwaterisexaminedinfabtotesttheirefecttogrowthofmyceliaofpomegranatewiltpathogen(Ceratocystisfimbriata)andmultiplicationofBacilluss“6．IResultsITheresultshowsthatgarlicrootexudateswhatevercultivatedinMSsolutionordistilledwatercouldnotinhibitorpromotemyceliagrowthofC．fimbria招．However．garlicrootex·udatescultivatedinbothmethodsefectivelypromotemultiplicationofB．subtilis．jConclusionsIItissuggestedthatintercroppinggarlicwithpomegranatetreebycombiningapplicationB．subtiliscouldbeapromisingwaytopreventpomegranatewiltspreadinpractice．KeywordsGarlic；Pomegranatewilt：Ceratocystisfimbriata；Bac~ussubtilis；RootexudatesPunicagranatumLinn．isoneofthetraditionalcharacter—Isticfruittrees．MengziCountyinYunnanProvincehasbeenoneofthesixIargestPunicagranatumLinn．productionareasandhasaplantationhistoryofmorethan800years．PunicagranatumLinn．istheadvantaqe0usproductionforIocalfarm—erstoearnmoremoney．TheplantationscaleisthetoponeinChina．Therefore．Mengziisnamedas”ChinesePunicagranatumLinn．County“l1J．AlongwiththeadvanceofMengzipomegranateindustry，theplantationareaisexpandingandtheageofthetreeisincreasing．Thediseasedisasterofpomegranateismoreandmoreserious．Among廿1em．thewitheredpomegranatecausedbyceratocystisfimbateisthemostseriousone．Pomegranatewithereddiseaseisonekindsendangeredandseriousdisastrousdisease，whichcouldre—suitedintodiedpomegranateinmorethantwoweeks．Thus．itmightresultinthedestructionoftheentirepomegranategar-denandseriouslyinfluencethedevelopmentofpomegranateIndustry．Thediseasedevelopsrapidlysince1999andresultsintoseriousIossestopomegranateproductionL2J．Sincere—portsonpomegranatewithereddisease．thoughsomeinvesti-gationsondiseaseoccurrenceregularityandpathogenidentifi—cationhavebeendone．therearenoreportsontheefec—tivemethodsoffielddisease—prevention-sj．AsadvantaaeOusagriculturaIproductsofhigheconomicvalueofhigh—gradefruitandearningforeignexchangethroughexport．non-chemicaImethodisappliedtocontroIpomegran．atewithereddiseaseanddecreasetheapplicationofagricu1．turaIdiseases．andplaysanimportantroleinenhancingpomegranatequailtyandeconomicoutcome．Inrecentyears．theantibacterialactionofbiocontrolbacteriasuchasBacillussubtilisshowsagoodpreventionoutcomejnmanyvegetationtungalpathogenssuchasFusar．umoxysponumt．curneri-n“mandco~onwithereddisease【．However．afterplant—ingbio—preventionbacteriaintosolI．Itcannoefectivelyes-tablishandproliferatebecauseofinfluencefrOmrootenvi—Received：December3，2010Accepted：January30，2011SuppoSedbyNational”973“Project(2011CB100400)．Correspondingauthor．E-mail：eastuptang@126．comrOnmentmulti-biologyandnon—biologicalfactors．showingin-consistencyoffieldpreventionoutcomeandindoorbiologicaImeasurement...
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