2011年2月第19卷第1期中国实验动物学报ACTALABORAT0RIUMANIMALISSCIENTIASINICAFebrua~，2011V0l_19NO．1单侧甲状腺电凝毁损法构建大鼠亚临床甲状腺功能减退症模型葛金芳，赵斌，彭磊，吴婷妮，解雪峰，胡成穆(安徽医科大学药学院，合肥230032)【摘要】目的评价单侧甲状腺电凝毁损法建立大鼠亚临床甲状腺功能减退症模型的可行性。方法36只Wistar大鼠随机分为假手术组、亚临床甲状腺功能减退症组和临床甲状腺功能减退症组。亚临床甲状腺功能减退症组大鼠电凝毁损单侧甲状腺，临床甲状腺功能减退症组大鼠电凝毁损全部甲状腺，假手术组暴露甲状腺而不毁损。饲养2周后，相同时问组间平行采血、化学发光法检测血清TSH、fT4、T3水平，光镜下观察亚临床甲状腺功能减退症组大鼠对侧甲状腺组织病理形态学变化。结果与假手术组比较，大鼠单侧电凝毁损甲状腺术后2周血清TSH水平轻度升高而T3、fT4则无显著变化，残留甲状腺组织轻度增生，符合亚临床甲状腺功能减退症的特征；大鼠双侧电凝毁损甲状腺2周后血清T3、fr4显著下降、TSH水平显著升高，符合临床甲状腺功能减退症的诊断标准。结论单侧甲状腺电凝毁损法建立大鼠亚临床甲状腺功能减退症模型是用于研究亚临床甲状腺功能减退症发生及发展机制的简便可靠模型。【关键词】亚临床甲状腺功能减退症，亚临床甲减，亚甲减；模型；甲状腺【中图分类号】Q95—33。R．33【文献标识码】A【文章编号】1005-4847(2011)01—0056．03Doi：10．3969／j．issn．1005-4847．2011．01．013Establishmentofaratmodelofsubclinicalhypothyroidismbyhemi-thyroidelectrOc0agulati0nGEJin—fang，ZHA0Bin，PENGLei，WUTing—ni，XIEXue—feng，HUCheng—mu(SchoolofPharmacy，AnhuiMedicalUniversity，Hefei230032，China)【Abstract】ObjectiveToinvestigatethefeasibilityofhemi—thyroidelectrocoagulationintheestablishmentofaratmodelofsubclinicalhypothyroidism(SCH)．MethodsThi~y-sixyoungWistarratswererandomlydividedintothreegroupsincludingsham，subclinicalhypothyroidism，andclinicalhypothyroidism(CH)groups．TheratsinSCHgroupweregivenhemi—thyroidelectrioc0agulati0n，ratsinCHgroupweregiventotalthyroidelectr0coagulation，whileratsintheshamgrouponlyexposedthyroidwithoutelectrocoagu1ation．2weeksafteroperation，bloodsamplesofallratswerecollectedandserumthyrotropic—stimulatinghormone(TSH)，freethyroxine(iT4)，andtriiodothyronine(T3)weredetectedbychemolu—minescence，whilethethyroidtissuespecimenswereexaminedbyhistopathologyusinghematoxylinandeosin(HE)stai-ning．ResultsComparedwiththeshamgroup，theweightofratsinCHgroupdecreased，whiletherewasnosignificantdiferencebetweentheSCHgroupandshamgroup．TheTSHlevelinbothSCHandCHgroupswassignificantlyincreased，theserumiT4andthiiodothyr0nineT3levelofCHratsdecreasedsignificantly，whiletherewasnosignificantdiferencebe—tweentheSCHgroupandshamgroup．ThepathologyrevealedslighthyperplasiainthecontralateralthyroidofSCHrats．ConclusionsTheestablishmentofSCHratmodelbyhemi—thyroidelectroc0agu1ationissimpleandconvenientforlongitu—dinalstudyonthemechanismofinitiationandprogressofsubclinicalhypothyroidism．【Keywords】Thyroid；Rat；Subclinicalhypothyroidism(SCH)；Model；Eleetroeoagnlation亚临床甲状腺功能减退症(亚临床甲减症，sub—clinicalhypothyroidism，SCH)，又称轻度甲状腺功能衰竭(mildthyroidfailure，MTF)，是临床甲状腺功能减退症(clinicalhypothyroidism，CH)发展的早期阶[基金项目】安徽省教育厅自然科学基金重点项目(编号：KJ2010A185)。[作者简介]葛金芳(1975一)，女，研究方向：分子药理学。E-mail：aygjf@mail中国实验动物学报2011年2月第19卷第1期ActaLabAnitaSciSin，February，2011，Vo1．19．No．157段，其特征为血清甲状腺素(thyroxine，tetraiodothyro．nine，T4)、游离甲状腺素(free...
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