贵州两种苦丁茶黄酮含量及抗氧化活性比较摘要黄酮是一类化合物的总称，它具有抑菌、抗炎、抗肿瘤、抗HIV、抗氧化、抗肿瘤等功效。在木姜子植物中，生物碱、黄酮类、甾体、木质素、内脂、挥发油等在医学研究上比较常用。黄酮易溶于乙醇等有机溶剂，根据相似相容原理，本实验采用乙醇热回流提法提贵州遵义余庆大叶苦丁茶和遵义余庆小叶苦丁茶（leafofchineseholly）中的总黄酮，以芦丁为对照品，以黄酮提取率和抗氧化活性进行试验，在试验的基础上通过测出贵州遵义余庆大叶苦丁茶和小叶苦丁茶黄酮含量的以及抗氧化活性的强弱，最终通过试验比较得出贵州两种苦丁茶总黄酮含量多少以及它们抗氧化的强弱。并采用紫外-可见分光光度法测定贵州两种苦丁茶提取液中总黄酮的含量，最后采用DPPH自由基清除率实验测定黄酮的抗氧化活性。得到试验结果如下:在大叶苦丁茶黄酮提取率为1.356%，小叶苦丁茶黄酮提取率为1.235%，大叶苦丁茶抗氧化活性为75.3，小叶苦丁茶抗氧化活性为73.6关键词：苦丁茶；回流提取，黄酮含量，抗氧化活性ABSTRACTFlavonoidsareaclassofcompounds,whichhaveantibacterial,anti-inflammatory,anti-tumor,antiHIV,anti-oxidation,anti-tumoreffect.Intheplant,thealkaloids,flavonoids,steroids,lignin,internalfat,volatileoilandsoonarecommonlyusedinmedicalresearch.Flavonoidssolubleinorganicsolventssuchasethanol,accordingtothesimilarityprincipleofcompatibility,theexperimentofextractingLitseaGuizhoubyethanolextraction(Litseacubeba(Lour.)Pers)totalflavonoids,rutin,theextractionrateofflavonoidsasindex,ethanolconcentration,solid-liquidratio,extractiontemperaturesinglefactortestandextractiontimeasfactors,theorthogonaltesttoinvestigatetheeffectsofethanolconcentration,extractiontime,extractiontemperatureandsolid-liquidratioontheextractionrateofflavonoidsonthebasisofsinglefactortest,finallythroughtheorthogonaltesttheoptimumextractionconditionsoftotalflavonoidsinGuizhousericea.UVvisiblespectrophotometrywasusedtodeterminethecontentof贵阳学院毕业论文（设计）totalflavonoidsintheextractofgingerandginger.ThefreeradicalscavengingactivityofflavonoidswasdeterminedbyDPPHfreeradicalscavengingrate.Theresultswereasfollows:theoptimumexperimentalconditionwas70%ethanolasextractant,theratioofmaterialtoliquidwasl:20,and2Hwasextractedatatemperatureof70DEGC,theextractionratewasupto1.252%,andtheantioxidantactivitywasabout78.9.Keywords:Litsea;flavonoids;refluxextraction;antioxidantactivity;1贵阳学院毕业论文（设计）目录1文献综述..................................................................................................................................41.1概述....................................................................................................................................41.1.1...................................................................................................................................41.1.2...................................................................................................................................51.2研究现状............................................................................................................................51.2.1...................................................................................................................................51.2.2...................................................................................................................................61.2.3..........................................................................................................................
