题目名称：菜心组织培养与离体再生研究目录标题....................................................................I摘要....................................................................I关键词....................................................................I1前言...................................................................12材料与方法..............................................................12.1试验材料..........................................................12.2试验设计..........................................................13结果与分析..............................................................23.1无菌苗的获得.......................................................23.2不同外植体对不定芽分化的影响......................................33.3不同激素配比对不定芽分化的影响....................................43.4不同生根培养基生根效果比较.........................................44讨论..................................................................45结论..................................................................5参考文献..................................................................5致谢....................................................................71菜心组织培养与离体再生研究摘要：为了获得菜心再生体系，以“四九”菜心、“60天”菜心种子为试验材料，对菜心进行组织培养与离体再生的研究。试验用两种菜心种子接种获得无菌幼苗，用它们的带叶柄子叶和下胚轴作为外植体，接种于不同激素配比的分化培养基上进行，再以获得的不定芽进行生根培养，获得了菜心离体再生体系。试验结果表明：“四九”菜心种子经消毒处理无菌接种后在光照光照强度为2250lx，光照时间16h，温度25℃下培养6天可获得无菌苗。带叶柄子叶为适宜的外植体；在MS+6-BA2.5mg/L+NAA0.25mg/L+30g/L蔗糖+8g/L琼脂的分化培养基进行一段时间的培养，结果表明：不定芽及愈伤组织的诱导率依次为95%、75.6%，诱导效果最佳；而生根培养在1/2MS+NAA0.15mg/L浓度组合下，培养效果最佳，MS培养基的生根效果次之。为菜心离体培养与再生研究提供了依据。关键词：菜心；外植体类型；激素配比；生根培养StudyonTissueCultureandInVitroRegenerationofChineseCabbageAbstract:InordertoobtaintheregenerationsystemoftheChinesecabbage,the"Forty-nine"Chinesecabbageandthe"60-day"ChinesecabbageseedswereusedasexperimentalmaterialstoconducttissuecultureandinvitroregenerationoftheChinesecabbage.Intheexperiment,twokindsofChinesecabbageseedswereusedtoinoculatesterileseedlings,andtheirpetiolecotyledonsandhypocotylswereusedasexplants,andtheywereinoculatedondifferentiationmediawithdifferenthormoneratios.Thentheadventitiousbudsobtainedwererootedandcultivate,ObtainedtheregenerationsystemofChinesecabbageheartinvitro.Thetestresultsshowedthataftersterileinoculationof"SiNine"Chinesecabbageseeds,sterileseedlingscanbeobtainedbyculturingatalightintensityof2250lx,alighttimeof16h,andatemperatureof25°Cfor6days.Cotyledonswithpetiolesaresuitableexplants;theywereculturedforaperiodoftimeonthedifferentiationmediumofMS+6-BA2.5mg/L+NAA0.25mg/L+30g/Lsucrose+8g/Lagar,theresultsshowed:adventitiousbudsTheinductionrateofcallusandcalluswas95%and75.6%,andtheinductioneffectwasthebest;whiletherootingcultureunderthecombinationof1/2MS+NAA0.15mg/Lconcentration,thecultureeffectwasthebest,andtherootingeffectofMSmediumwasthesecond.ItprovidesabasisfortheresearchoninvitrocultureandregenerationofChinesecabbage.Keywords:Chinesecabbage;explanttype;hormoneratio;rootingculture21前言菜心（BrassicacampestrisL.）属芸薹属白菜...
