325吨/年硫酸双氢链霉素生产装置工艺设计程伟（辽宁石油化工大学，环境与生物工程学院，生物工程0901，辽宁抚顺，113001）摘要本课题为325吨/年硫酸双氢链霉素生产装置的工艺设计。在发酵过程中，每天需完成至少51.055m3（53607.75kg）发酵全液的生产。经计算，生产中选用8个75m3的发酵罐，发酵周期为8d，一、二、三级种子罐各4个，发酵周期为4d。菌种经一、二、三级种子罐逐级扩大培养后放罐入发酵罐中，在发酵罐中经8d发酵，产成51.055m3的发酵全液，后放入预处理罐内进行硫酸双氢链霉素的合成提取。发酵液进入预处理罐后，用草酸酸化至pH3.0左右，通过板框过滤，除去大量杂质，再用NaOH中和至pH在6.7~7.2范围内。离开预处理罐的发酵液进入稀释罐进行稀释，后进入交换罐。在交换罐中采用110¿3Na型树脂对稀释液进行吸附，再经7%H2SO4溶液进行解吸，解吸完毕，采用活性炭粉对解吸液脱色。解吸后的解吸液在补还原罐中用NaBH4将硫酸链霉素还原为硫酸双氢链霉素，还原后的硫酸双氢链霉素经稀释、吸附、解吸精制、精脱、薄膜蒸发、成脱和离心喷雾干燥等步骤形成成品。关键词：硫酸双氢链霉素；生产装置；工艺流程；设计Atechnologicaldesignof325t/adihydrostreptomycinsulphateproductionfacilityChengWei(Class0901,Departmentofbiologicalengineering,SchoolofEnvironmentalandbiologicalengineering,LiaoningShihuaUniversity,LiaoningFushun,113001,China)AbstractThistopicisthetechnologicaldesignof325t/adihydrostreptomycinsulphateproductionfacility.Inthefermentationprocess,ithavetocompleteatleast51.055m3(53607.75kg)fermentationliquidproductioneveryday.Aftercalculation,theresultsisthattheproductiondesignselecteeight75m3fermenter,thefermentationperiodwas8d,the1st,2nd,3rdseedtankisfour,respectively.After8dfermentation,51.055m3fermentationbrothisobtained.Thenthefermentationbrothisputintothepretreatmenttankforthesynthesisofdihydrostreptomycinsulfateextraction.Afterthefermentationbrothintothepretreatmenttanks,itisacidifiedtonearlypH3.0withoxalicacid.Thenitcanremovelargeamountsofimpuritiesthroughtheplateandframefilter,anditisneutralizedtoapHrangeof6.7to7.2withNaOH.Afterfermentationbrothleavingthetankintothedilutiontankfordilution,thefermentationbrothflowsintotheexchangetank.Inexchangetank,ituses110¿3Natyperesinforadsorptionondilution.Thenthedilutionisdesorbedcompletelyby7%H2SO4solution.Afterdesorption,desorptionsolutionisdecoloredusingactivatedcarbonpowder.Afterdesorption,thestreptomycinsulfateindesorptionsolutionwillbereducedtodihydrostreptomycinsulfateusingNaBH4inthereductionpot.Afterreduced,dihydrostreptomycinsulfateformsfinishedgoodsthroughdilution,adsorption,desorptionrefined,lossofessence,athinfilmevaporator,acentrifugalspraydryingoffandothersteps.Keywords:Dihydrostreptomycin,Productionfacility,Process,Design目录摘要..................................................................................................................IAbstract.............................................................................................................................II第1章绪论...............................................................................................11.1链霉素简介....................................................................................11.2国外生产和技术水平现状........................................31.3国内生产和技术水平现状........................................31.4生产技术的展望......................................................................41.5链霉素的生产制备..............................................................41.6链霉素的用途......
