摘要本实验选取多肉植物宝草锦（Haworthiacymbiformisvar.triebnetpoelln）的不定芽为实验材料，通过MS培养基与不同浓度激素的配比处理宝草锦不定芽，旨在建立一种方便快捷且高效的宝草锦快速繁殖体系，为其种质资源保护、开发利用、园艺栽培、遗传转化及分子育种等提供参考。首先选取宝草锦无污染不定芽在超净工作台进行接种，然后接种到不同激素浓度配比的MS培养基中进行增殖培养。实验结果表明：诱导宝草锦不定芽增殖的最适培养基是：MS+6-BA1mg/L+NAA0.1mg/L。关键词宝草锦；组织培养；不定芽；快繁技术StudyontheOptimizationofCultureHormoneinSubcultureofHaworthiaCymbiformisVar.triebnetPoellnAbstractInthisstudy,theadventitiousbudsofHaworthiacymbiformisvar.triebnetpoellnwereusedasexperimentalmaterials.Inordertocomparetheoptimumconditionsforthestudyoftheprocessofthemultiplicationofthebudandtheadventitiousbudsofthebrocade,theMSculturemediumandthedifferentconcentrationofhormoneswereusedtotreatthebudofthebrocadeandbrocade.Theaimistoestablishaconvenientandefficientsystemforrapidpropagationofbroccoli.Itcanprovidereferenceforgermplasmresourcesprotection,developmentandutilization,horticulturalcultivation,genetictransformationandmolecularbreeding.First,thepollution-freeadventitiousbudsoftheHaworthiacymbiformisvar.triebnetpoellnwereinoculatedinthesupercleanworktable,andtheninoculatedintoMSmediumwithdifferenthormoneconcentrationtoproliferate.TheresultsshowedthattheoptimummediumforinducingproliferationofadventitiousbudswasMS+6-BA1mg/L+NAA0.1mg/L.KeywordsHaworthiacymbiformisvar.triebnetpoelln,tissueculture,adventitiousbud,rapidpropagation目录1前言.........................................................................................................................................11.1植物组织培养外植体的选用消毒..................................................................................11.2植物组织培养培养基和培养方式的选用.......................................................................11.2.1培养方式的选用.......................................................................................................11.2.2培养基的选用............................................................................................................21.3培养基的培养条件...........................................................................................................21.4不定芽增殖所需植物激素的比例...................................................................................21.5宝草锦的概述...................................................................................................................31.6研究意义...........................................................................................................................32材料与方法...................................................................................................................4........................................................................................................................................32.1主要实验材料、试剂和设备...........................................................................................42.1.1实验材料....................................................................................................................42.1.2实验试剂....................................................................................................................42.1.3实验所用培养基................................
