摘要刺玫果学名野蔷薇,又名野刺玫果,山刺玫果,系蔷薇科蔷薇属植物山刺玫的成熟果实。广泛分布于我国的东北地区，尤以黑龙江省贮量最为丰富。现代药理学研究表明：刺玫果含有丰富的营养成分及生物活性物质，具有健脾理气、养血调经、抗衰老、防治心血管疾病、增强免疫及促智等功效。刺玫果中所含的总黄酮类物质具有预防和治疗高血脂、血栓所致的心脑血管疾病的功效，且具有高效、低毒副作用的优势。因此纯化刺玫果中的总黄酮就具有现实的科研意义。目前，常见的总黄酮纯化方法有水提醇沉法（水醇法）、醇提水沉法（醇水法）、酸碱法、盐析法、离子交换法和结晶法等。实践表明，这些方法也存在一定的局限性。近年出现了一些分离和纯化的新方法，如絮凝沉淀法，大孔树脂吸附法、超滤法、高速离心法等。本论文采用大孔树脂法纯化刺玫果提取物中的总黄酮。探索洗脱剂、pH值、上样浓度等因素对纯化过程的影响。采用紫外分光光度计法对总黄酮含量进行了分析，最终确定了洗脱剂的浓度75%、上样PH为5，上样浓度为原液浓度，吸附速率为2Bv/h，洗脱速率为1Bv/h。该纯化方法简单，快捷，纯化效率高，进一步拓展了刺玫果的应用领域和范围，为刺玫果的全面和综合利用提供科学的理论依据。关键词：刺玫果总黄酮大孔树脂法纯化AbstractRosamultifloraisisthescientificnameofRosadavuricapall，alsoknownasWildrosafruit.Rosamountainfruit,thisfruitistheRosamountainfruitofRosaceaegenusRosa.Itisgrowingineast-northChina,especiallyinprovinceofHeilongjiang.ModernpharmaceuticalresearchshowsthatRosadavuricaPallcontainsrichnutrientsandbioactivesubstances,anti-aging,preventionandtreatmentofcardiovasculardisease,enhancesimmunityandtheefficacyofnootropic,etc.TotalflavonoidsintheRosadavuricaPallpossessthefunctionofpreventionandtreatmentofhighbloodlipids,thrombosisduedtocardiovascularandcerebrovasculardiseases.Ithaspropertieslikehigh-efficiencyandtheadvantagesoflowtoxicitysideeffects.Thusitisworthindoingresearchonrefiningtotalflavonoidsinthefruit.Recently,therearetheseveralcommonmethodsinpurifyingFlavonoidssuchaswater-extractingalcohol-precipitating,alcohol-extractingwater-precipitating,acid-basemethod,saltdopositing,io-exchangeandcrystallization,andsoon.Inpractice,thereisthelimitinallthesemethodreported.Somenewmethodsareinvestigatedlikeflocculationprecipitation,macroporousresinabsorption,ultrafiltration,high-speedcentrifugea-tion,andsoon.Inthisthesis,wereportextractionoftotalflavonoidsfromRosadavuricapallbyusingthemethodofMacroporousresin.ItwasinvestigatedbasingonhowthetypeofEluant,PHofsolution,concentrationofsamplesinfluencespurification.UsingUltravioletspectrophotometermethodtoanalyzeFlavonoids,ThismethodistoknowthecontentoftotalFlavonoidsbymeasuringUltravioletabsorption.Asaresult,itwasfoundthattheconcentrationofEluantis75%ethanol,pHis5andthesampleconcentrationisconcentrationoforiginalsolutionl,Adsorptionrateis2Bv/h,Elutionrateis1Bv/h.Thismethodissimple,rapidandhigh-efficient.ThisresearchhaveobtainedsomescientificresultswhichwillboardtheapplicationofRosadavuricapall.Keywords：RosadavuricapallTotalFlavonolMacroporousresinpurification目录摘要........................................................................................................................IABSTRACT.............................................................................................................II1绪论....................................................................................................................11.1课题背景..................................................................................................
