关于食用菌工厂化生产污染菌的研究摘要从杏鲍菇工厂中患病的杏鲍菇上分离到了2个菌株（指定为S1，S2），接种至种包中，产生了同样的病变。根据革兰氏染色和生化检验结果，初步鉴这两个菌株为假单胞菌属。进一步对其生理生化特性，分析16SrRNA序列，鉴定S1为Pseudomonasputida，S2为Pseudomonasmarginais。关键词：生化检验杏鲍菇16SrRNA基因PseudomonasputidaPseudomonasmarginaisAbstractInplacesofproductionbacteriawererepeatedlyisolated.Fromtheselesionstwobacterialstrains(designatedS1,S2)vaccinationtothepackages,thenproducethesamekindofillness.ResultsofGramstainandbiochemicaltestspreliminarilyidentifiedtheseisolatesasPseudomonas.Physiologicalandbiochemicalproperties,analysisofthe16SrRNAsequences,identifiedS1asPseudomonasputida.Pseudomonas,andS2asPseudomonasmarginais.KeywordsFlammulinavelutipes.PleurotusEryngii.Pseudomonasputida.Pseudomonasmarginais.16SrRNAgene.目录1.引言...........................................................11.1课题研究背景........................................................................................................................11.2课题国外研究状况................................................................................................................11.3常见细菌病害........................................................................................................................21.4课题主要研究内容................................................................................................................22.材料与方法....................................................32.1主要仪器和培养基................................................................................................................32.1.1主要仪器.........................................................................................................................32.1.2主要培养基.....................................................................................................................32.2病原菌的分离纯化................................................................................................................42.3病原菌的形态观察及染色....................................................................................................72.4病原菌的生理生化性状........................................................................................................72.4.1葡萄糖氧化发酵作用的测定.........................................................................................32.4.2氧化酶.............................................................................................................................42.4.3色素的产生.....................................................................................................................42.4.4接触酶.............................................................................................................................42.4.5甲基红试验（M.R.试验）.............................................................................................52.4.6乙酰甲基甲醇试验（V.P.试验）...................................................................................52.4.7产生吲哚试验.................................................................................................................52.4.8水解七叶灵.....................................................................................................................62...
