鸡的大小黄白卵泡分离及总RNA的提取目录中文摘要：................................................................................................2关键词：....................................................................................................2Abstract：.................................................................................................2前言............................................................................................................21.材料和方法............................................................................................41.1实验动物及其组织.......................................................................41.2主要仪器和试剂...........................................................................41.2.1实验仪器.............................................................................41.2.2实验试剂.............................................................................41.2.3溶液配制.............................................................................51.3实验操作步骤...............................................................................51.3.1取样.....................................................................................51.3.2大小黄白卵泡的提取.........................................................61.3.3总RNA的提取.....................................................................61.3.4总RNA完整性鉴定..............................................................61.3.5总RNA内基因组DNA的去除.............................................61.3.6反转录反应.........................................................................71.3.7PCR反应.............................................................................71.3.8电泳检测............................................................................71.3.9PCR产物纯化.....................................................................71.3.10连接转化..........................................................................82.实验结论..........................................................................................82.1鸡卵泡颗粒细胞..........................................................................82.2总RNA的检测结果......................................................................92.3PCR检测.....................................................................................93.分析讨论..............................................................................................104.参考文献..............................................................................................12中文摘要：实验前,在大量查阅各项相关研究资料的前提下，明确了CART与蛋鸡卵巢中不同大小黄白卵泡中的雌激素（Estrodiol,E2）和孕酮（P）分泌量的关系。本实验运用显微外科手术技术分离蛋鸡的大小黄卵泡、大小白卵泡，并分别进行总RNA的提取，通过CARTmRNA表达量的差异对CART蛋白进行定位，再通过体外注射dsRNA对CART的表达进行抑制，阻断CART对蛋鸡卵巢中卵泡生长发育及排卵的影响，从而提高蛋鸡产蛋量，延长产蛋周期，降低料蛋比，进而通过饲养试验，确定调控效果，并将该技术进行全面示范推广。关键词：CART；卵泡；总RNA；dsRNA；Abstract：Accordingtotheinformationofrelatedresearchbeforeexperiments,wefoundouttherelationshipsbetweentheamountofestrogen(E2)andprogesterone(P),whichthelayer’sfollicleindifferentsizesecreted,andCART.Duringthisexperiment,weusedthemicrosurgicaltechniq...
