I狗牙根改善胰岛素抵抗活性部位的筛选目 录摘要......................................................................................................................I-II1 前言......................................................................................................................12 材料与仪器..........................................................................................................32.1 实验细胞..........................................................................................................32.2 药品与试剂......................................................................................................32.3 主要仪器..........................................................................................................42.4 试剂配制..........................................................................................................43 实验方法..............................................................................................................53.1 HepG2 细胞体外常规培养..............................................................................53.2 HepG2 细胞胰岛素抵抗模型的建立..............................................................63.3 WST-1 法检测狗牙根各萃取部位对HepG2细胞增殖活性的影响..............73.4 狗牙根各萃取部位对IR-HepG2 细胞葡萄糖消耗的影响..........................83.5 统计分析..........................................................................................................94 结果......................................................................................................................94.1 胰岛素对HepG2细胞增值的影响..................................................................94.2 胰岛素对HepG2细胞葡萄糖消耗的影响......................................................94.3 狗牙根各萃取部位对正常HepG2细胞增殖活性的影响..............................104.4 狗牙根各萃取部位对IR-HepG2 细胞葡萄糖消耗的影响..........................115 讨论...................
