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可口革囊星虫盐度胁迫体腔液

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I摘 要为了探讨可口革囊星虫(Phasolosma esculenta)耐受低盐下的反应机制, 对照组为自然海水, 研究在低盐度胁迫下星虫的致死率,及胁迫0h、3h、6h、12h、24h 和 48h 时体腔液的 Na+、K+、Cl-的浓度和肠、体壁组织的生理指标、组织学的变化。结果表明,随着盐度从 10ppt 下降到 5ppt,可口革囊星虫的致死率逐渐升高。在盐度 10ppt 作用下,星虫血液的 Na+、K+和 Cl-的浓度快速下降,而后维持在一定值。肠壁和体壁 Na+/K+-ATP 酶(NKP)和酸性磷酸酶(ACP)活性分别在 6h 和12h 达到高峰,而后稳定在略高于对照组的水平。在盐分胁迫下,肠和体壁的结缔组织细胞变大而疏松。 研究表明,低盐胁迫下酶系统和非酶系统发生了变化,为揭示低盐胁迫反应的机理提供了部分的线索。关键词:可口革囊星虫;盐度;胁迫;体腔液 IIAbstract In order to investigate the response mechanism of Phasolosma esculenta to low salinity, the control group was natural seawater. The mortality rate of Phasolosma esculenta under low salinity stress was studied, as well as the concentration of Na+, K+ and Cl- in the body cavity fluid and the physiological and histological changes of intestinal and body wall tissues under 0h, 3h, 6h, 12h, 24h and 48h stress. The results showed that with the decrease of salinity from 10ppt to 5ppt, the mortality rate of dendrosaceae was gradually increased. Under the action of a salinity of 10ppt, the concentrations of Na+, K+ and Cl- in starworm blood decreased rapidly and then remained at a certain value. The activity of Na+/K+ -atpase (NKP) and ACP in intestinal wall and body wall reached a peak at 6h and 12h respectively, and then stabilized at a level slightly higher than that in the control group. Under salt stress, the connective tissue cells of the intestine and body walls become large and loose. The results showed that the enzyme system and non-enzyme system changed under low salt stress, which provided some c...

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