摘 要酮洛芬作为一种抗炎药被广泛应用在医药学领域中，因其具有生物累积性而在环境中长期存在。本实验选择酮洛芬(Ketoprofen，简称 KP)和锦鲫作为实验材料，在室内模拟条件下进行半静态染毒实验，研究低浓度 10 mg/kg、高浓度 100 mg/kg 的酮洛芬对锦鲫肝脏抗氧化防御系统的影响。实验结果：在 KP 10mg/kg 时，锦鲫的 SOD 酶活性均受到抑制，CAT 酶活性先抑制后诱导上升，这说明锦鲫的抗氧化防御系统损伤并不明显；在 KP100mg/kg 时，锦鲫 SOD 酶活性均受到抑制，CAT 酶活性先诱导后抑制下降，这说明了锦鲫的抗氧化损伤不明显。这表明了更高浓度的 KP 或者更长的暴露时间才会对锦鲫的SOD、CAT 酶活性起到显著的抑制作用，但这些体内表征的变化都说明了酮洛芬会造成锦鲫肝脏的氧化损伤。关键词：锦鲫；抗氧化防御系统；酮洛芬；SOD；CATAbstractKetoprofen is widely used as an anti inflammatory drug in the field of medicine and has long been found in the environment due to its bioaccumulation. Ketoprofen Ketoprofen Ketoprofen Ketoprofen (KP) and Carassius Auratus Gibelio were selected as experimental organisms to carry out semi-static toxicity experiments under indoor simulated conditions, the effects of Ketoprofen at low concentration (10mg / kg) and high concentration (100mg / kg) on antioxidant defense system in liver of Carassius auratus were studied. Results: When KP was 10mg / kg, the activity of SOD in Carassius auratus was inhibited, and the activity of CAT in Carassius auratus was induced to increase, which indicated that the antioxidant defense system of Carassius auratus was not damaged obviously, cat Activity was first induced and then decreased, which indicated that the antioxidant damage of Carassius auratus was not obvious. This indicated that higher concentration of KP or longer exposure time could significantly inhibit the activity of SOD and CAT in Carassius auratus, but these changes of in Vivo characterization suggested...
