外源基因转化烟草悬浮细胞的研究摘要烟草悬浮细胞作为一种在生命科学领域研究比较广泛的实验材料。烟草悬悬浮细胞具有选材便捷、传代速度较快的优良特性，便于实验室操作和培养。通过把外源基因进行克隆和载体构建后转移到烟草悬浮细胞中，利用抗生素进行筛选，培养在固体培养基中，等到得到合适的愈伤组织后，再把愈伤组织转移到液体培养基中培养，利用农杆菌的介导作用筛选出含有外源基因的细胞，把含有外源目的基因的愈伤组织夹碎后放在含有培养液的锥形瓶中培养，把锥形瓶放到摇床上就能得到悬浮细胞。本文主要综述在实验室条件下通过农杆菌的介导、筛选出外源基因在烟草悬浮细胞中的转化及其烟草悬浮细胞的制备。关键字：农杆菌的介导；外源基因的转化；烟草悬浮细胞的制备Study on the transformation of tobacco suspension cells by exogenous genesStudent: qian hong instructor: feng hanqing(college of life science, northwest normal university, lanzhou 730000, China)Abstract：Tobacco suspension cells are widely used as experimental materials in the field of life science.Tobacco suspension cells have the advantages of convenient material selection and fast passage speed, which is convenient for laboratory operation and culture.Through the exogenous gene cloning and transferred to the tobacco suspension cells after carrier construction, use of antibiotics screening, culture in solid medium, wait to get suitable callus, the callus again transferred to liquid medium culture, make use of agrobacterium mediated screen contains cells of the exogenous gene, the contain of the outside source purpose gene callus after minced in the conical flask containing nutrient solution culture, the conical flask on the table will be suspended cells.This paper mainly reviews the transformation of exogenous genes in tobacco suspension cells and the preparation of tobacco suspension cells by agrobacterium-mediated screening under laboratory conditions.Key words: Agrobacterium-mediat...
