摘 要本文以单壁碳纳米管(SWCNTs)为研究对象，锦鲫为受试对象，采用实验室半静态染毒方法，分析暴露在不同浓度(10μg/L、100μg/L、1000μg/L)的单壁碳纳米管溶液在 7、14、21、28 天后鱼体肝脏内蛋白浓度、SOD 活性和 CAT 活性等指标，探讨其潜在的致毒机制。实验结果表明，与空白组对比，SWCNTs 高低浓度对蛋白质含量没有显著性影响；在实验前期，实验浓度为 10ug/L 的 SWCNTs 没有明显的影响到 CAT 活性和 SOD 活性，但是随着时间的增加，在 28 天后对CAT、SOD 活性都出现抑制现象；100ug/L 的 SWCNTs 除了在暴露第 7 天 SOD 活性和暴露 14 天 CAT 活性受到轻微诱导外，其他时候的酶活性都受到不同程度的抑制；随着浓度和染毒暴露时间的增加，1000ug/L 高浓度组的 SOD 活性均受到抑制，肝脏也有不同程度的损伤，CAT 活性也受到抑制，而且染毒时间越长，效果越明显。关键词：锦鲫；SOD；CAT；单壁碳纳米管Abstract The potential toxicogenic mechanism of single-walled carbon nanotube solution exposed to different concentrations (10μg/L、100μg/L、1000μg/L) after 7,14,21 and 28 days in the liver of fish was investigated by using the laboratory semi-static method using single-walled carbon nanotube (SWCNTs) as the subject. Compared with the blank group, the SWCNTs concentration had no significant effect on protein content. Prior to the experiment, the SWCNTs with 10 ug/L did not significantly affect the CAT activity and SOD activity, but with the increase of time, the inhibition of CAT、SOD activity appeared after 28 days.The SWCNTs activity of 100 ug/L was inhibited to varying degrees except SOD slight induction on day 7 and 14. With the increase of concentration and exposure time, the SOD activity of 1000 ug/L high concentration group was inhibited, and the liver was damaged to varying degrees, and the CAT activity was also inhibited, and the longer the exposure time was, the more obvious the effect was.Keywords：carassiusauratus;SOD;CAT;single...
