精品文档---下载后可任意编辑NO 在大豆与大豆花叶病毒互作过程中的变化特征及其功能初探的开题报告摘要：大豆花叶病毒（Soybean mosaic virus，SMV）是一种引起重要农作物大豆叶片病毒性病害的病毒。为了讨论 SMV 与大豆的互作机制，本讨论以大豆品种 Williams 82 为材料，通过比较 SMV 感病（S）和抗病（R）品种的组织学、生化学以及分子生物学特征的变化，初步探讨SMV 的致病机理和抗病机制。结果显示，SMV 可导致大豆组织内MDA、CAT 和 POD 三个抗氧化酶活性明显下降，丙二醛（MDA）含量和离子渗漏率明显上升，表明 SMV 感染引起了大豆细胞膜损伤和氧化应激反应，从而导致抗病性降低。在分子水平上，与健康大豆相比，SMV感染后，大豆 NO 含量明显升高，可参加一系列信号转导和抗病反应的调控。实验结果为讨论 SMV 与大豆互作机制奠定了基础。关键词： 大豆花叶病毒；抗氧化酶；氧化应激反应；NO；抗病性Abstract:Soybean mosaic virus (SMV) is a virus that causes significant viral diseases in soybean leaves. In order to study the interaction mechanism between SMV and soybean, this study used the soybean variety Williams 82 as the material. By comparing the histological, biochemical, and molecular biological characteristics of SMV-susceptible (S) and resistant (R) varieties, and preliminarily exploring the pathogenic mechanism and disease resistance of SMV. The results showed that SMV can cause the activity of three antioxidant enzymes MDA, CAT, and POD to decrease significantly in soybean tissues, and the content of malondialdehyde (MDA) and ion leakage rate to increase significantly, indicating that SMV infection causes damage to soybean cell membranes and oxidative stress reaction, leading to a decrease in disease resistance. At the molecular level, compared with healthy soybean, after SMV infection, the content of NO in soybean significantly increased and can participate in a series of signal transduction and disease resistance regulation. The experimental results have laid a foundation for the study of the interaction mechanism between SMV and soybean.Keywords: Soybean mosaic virus; Antioxidant enzyme; Oxidative stress reaction; NO; Disease resistance
